rab11 mcherry Search Results


rab11 mcherry  (Addgene inc)
88
Addgene inc rab11 mcherry
Rab11 Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rab11 mcherry/product/Addgene inc
Average 88 stars, based on 1 article reviews
rab11 mcherry - by Bioz Stars, 2026-05
88/100 stars
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sspb(micro)-mvenus-gcn4–ppkin14-vib(861–1321)_p2a_ilid-mcherry-rab11  (Polysciences inc)
90
Polysciences inc sspb(micro)-mvenus-gcn4–ppkin14-vib(861–1321)_p2a_ilid-mcherry-rab11
Disrupting kinesin-3 dimerization prevents spatial segregation between motor and cargo . Related to . (A) Design of kinesin-3 constructs. Monomeric and constitutively dimerized versions were engineered by deletion of the neck coil (aa 366–383) of KIF1A(1–383)-GFP-SSPB or replacement of the neck coil with the leucine zipper <t>GCN4.</t> (B and C) Fixed-cell imaging (B) and quantification (C) of KIF1A-GFP-SSPB variants and <t>mCherry-RAB11</t> in COS-7 cells. Quantification (C) shows the NPF of intensity. Dots represent one cell and bars indicate mean and 95% confidence intervals. Dotted lines represent the mean and the mean ± two times the SD of RAB11 distribution. Data from three independent experiments. Scale bars are 10 µm.
Sspb(micro) Mvenus Gcn4–Ppkin14 Vib(861–1321) P2a Ilid Mcherry Rab11, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sspb(micro)-mvenus-gcn4–ppkin14-vib(861–1321)_p2a_ilid-mcherry-rab11/product/Polysciences inc
Average 90 stars, based on 1 article reviews
sspb(micro)-mvenus-gcn4–ppkin14-vib(861–1321)_p2a_ilid-mcherry-rab11 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


Disrupting kinesin-3 dimerization prevents spatial segregation between motor and cargo . Related to . (A) Design of kinesin-3 constructs. Monomeric and constitutively dimerized versions were engineered by deletion of the neck coil (aa 366–383) of KIF1A(1–383)-GFP-SSPB or replacement of the neck coil with the leucine zipper GCN4. (B and C) Fixed-cell imaging (B) and quantification (C) of KIF1A-GFP-SSPB variants and mCherry-RAB11 in COS-7 cells. Quantification (C) shows the NPF of intensity. Dots represent one cell and bars indicate mean and 95% confidence intervals. Dotted lines represent the mean and the mean ± two times the SD of RAB11 distribution. Data from three independent experiments. Scale bars are 10 µm.

Journal: The Journal of Cell Biology

Article Title: An optimized toolbox for the optogenetic control of intracellular transport

doi: 10.1083/jcb.201907149

Figure Lengend Snippet: Disrupting kinesin-3 dimerization prevents spatial segregation between motor and cargo . Related to . (A) Design of kinesin-3 constructs. Monomeric and constitutively dimerized versions were engineered by deletion of the neck coil (aa 366–383) of KIF1A(1–383)-GFP-SSPB or replacement of the neck coil with the leucine zipper GCN4. (B and C) Fixed-cell imaging (B) and quantification (C) of KIF1A-GFP-SSPB variants and mCherry-RAB11 in COS-7 cells. Quantification (C) shows the NPF of intensity. Dots represent one cell and bars indicate mean and 95% confidence intervals. Dotted lines represent the mean and the mean ± two times the SD of RAB11 distribution. Data from three independent experiments. Scale bars are 10 µm.

Article Snippet: U2OS cells were transfected with iLID-mCherry-RAB11, KIF1A(1–365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11 or SSPB(micro)-mVenus-GCN4–ppKin14-VIb(861–1321)_P2A_iLID-mCherry-RAB11 using polyethylenimine (Polysciences).

Techniques: Construct, Imaging

Minus end–directed organelle redistribution using moss-derived kinesin-14. (A) Fixed-cell imaging of recycling endosomes (middle) tagged with FKBP-mCherry-RAB11 and the dynein adaptor FRB-BICDN-HA (top) in COS-7 cells, with or without rapalog (100 nM, 1-h treatment). (B) Moss-derived kinesin-14 VIb constructs. Addition of GCN4 leucine zipper creates a dimer of dimers, effectively creating a tetrameric motor. (C) Fixed-cell imaging of kinesin-14 VIb constructs in COS-7 cells. (D) Similar to A, but with cells expressing dimeric FRB-GFP–ppKin14-VIb. (E) Assay: early endosomes were tagged with iLID-mCherry-RAB5. Blue light illumination induced unfolding of iLID and binding to SSPB-GFP–ppKin14-VIb or SSPB-GFP-GCN4–ppKin14-VIb, stimulating anterograde transport (gray). (F) Quantification of live-cell imaging of iLID-mCherry-RAB5 and opto-kinesin KIF1A(1–365)-VVDfast-mVenus-SSPB(micro) in U2OS cells treated with nocodazole (10 µM) to depolymerize microtubules. Cells were illuminated continuously for 30 s with 100 µW cm −2 of blue light, followed by 150 s of darkness. Graph shows normalized intensity of opto-kinesin on individual endosomes. (G) Live-cell imaging and quantifications (H–K) of iLID-mCherry-RAB5 in U2OS cells expressing the indicated constructs before or after 10 min of illumination. Quantifications in H–K show peripheral enrichment index (H) or perinuclear enrichment index (I) of iLID-mCherry-RAB5. Plots in J and K show per-cell change in peripheral (J) or perinuclear (K) enrichment index after 10 min. Dots represent one cell and bars indicate mean and 95% confidence intervals. Dotted lines represent the mean of control cells. Boxed numbers show the percentage of responsive cells for each dataset (peripheral or perinuclear enrichment greater than mean + two times the SD of control). Graphs represent mean and 95% confidence intervals of at least 15 cells from a total of at least three experiments. Blue box indicates 470-nm illumination. Scale bars are 10 µm.

Journal: The Journal of Cell Biology

Article Title: An optimized toolbox for the optogenetic control of intracellular transport

doi: 10.1083/jcb.201907149

Figure Lengend Snippet: Minus end–directed organelle redistribution using moss-derived kinesin-14. (A) Fixed-cell imaging of recycling endosomes (middle) tagged with FKBP-mCherry-RAB11 and the dynein adaptor FRB-BICDN-HA (top) in COS-7 cells, with or without rapalog (100 nM, 1-h treatment). (B) Moss-derived kinesin-14 VIb constructs. Addition of GCN4 leucine zipper creates a dimer of dimers, effectively creating a tetrameric motor. (C) Fixed-cell imaging of kinesin-14 VIb constructs in COS-7 cells. (D) Similar to A, but with cells expressing dimeric FRB-GFP–ppKin14-VIb. (E) Assay: early endosomes were tagged with iLID-mCherry-RAB5. Blue light illumination induced unfolding of iLID and binding to SSPB-GFP–ppKin14-VIb or SSPB-GFP-GCN4–ppKin14-VIb, stimulating anterograde transport (gray). (F) Quantification of live-cell imaging of iLID-mCherry-RAB5 and opto-kinesin KIF1A(1–365)-VVDfast-mVenus-SSPB(micro) in U2OS cells treated with nocodazole (10 µM) to depolymerize microtubules. Cells were illuminated continuously for 30 s with 100 µW cm −2 of blue light, followed by 150 s of darkness. Graph shows normalized intensity of opto-kinesin on individual endosomes. (G) Live-cell imaging and quantifications (H–K) of iLID-mCherry-RAB5 in U2OS cells expressing the indicated constructs before or after 10 min of illumination. Quantifications in H–K show peripheral enrichment index (H) or perinuclear enrichment index (I) of iLID-mCherry-RAB5. Plots in J and K show per-cell change in peripheral (J) or perinuclear (K) enrichment index after 10 min. Dots represent one cell and bars indicate mean and 95% confidence intervals. Dotted lines represent the mean of control cells. Boxed numbers show the percentage of responsive cells for each dataset (peripheral or perinuclear enrichment greater than mean + two times the SD of control). Graphs represent mean and 95% confidence intervals of at least 15 cells from a total of at least three experiments. Blue box indicates 470-nm illumination. Scale bars are 10 µm.

Article Snippet: U2OS cells were transfected with iLID-mCherry-RAB11, KIF1A(1–365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11 or SSPB(micro)-mVenus-GCN4–ppKin14-VIb(861–1321)_P2A_iLID-mCherry-RAB11 using polyethylenimine (Polysciences).

Techniques: Derivative Assay, Imaging, Construct, Expressing, Binding Assay, Live Cell Imaging

Application of the optimized optogenetic toolbox to populations of cells. (A) Constructs: use of a P2A self-cleaving peptide enables expression of iLID-mCherry-RAB11 with SSPB-Kin14-VIb or opto-kinesin-SSPB at equimolar levels from a single construct. (B) Immunoblot (IB) of whole-cell lysates from U2OS cells expressing the indicated proteins. Dotted lines indicate the excision of lanes. Exp. MW, expected molecular weight. (C–F) Fixed-cell imaging (C) and quantification (D–F) of iLID-mCherry-RAB11 and opto-kinesin KIF1A(1–365)-VVDfast-GFP-SSPB(micro) from a single plasmid in COS-7 cells in the dark or after illumination with blue light (100 µW cm −2 ). (D) Quantification showing the NPF of RAB11 intensity. (E) Graph showing normalized cellular expression levels of KIF1A(1–365)-VVDfast-GFP-SSPB(micro) and iLID-mCherry-RAB11 when expressed from a single plasmid (magenta) as in A or using two separate constructs for motor and cargo (gray). (F) Graph showing normalized cellular expression levels of KIF1A(1–365)-VVDfast-GFP-SSPB(micro) and the peripheral fraction of RAB11 per cell in the dark (gray) and after illumination (blue). (G and H) Fixed-cell imaging (G) and quantification (H) of iLID-mCherry-RAB11 and SSPB(micro) in COS-7 from a single plasmid in COS-7 cells in the dark or after illumination with blue light (100 µW cm −2 ). Graph in H shows the normalized perinuclear fraction of RAB11 intensity (mean RAB11 MTOC intensity/mean cellular RAB11 intensity). (I) Live-cell imaging of iLID-mCherry-RAB11 in HeLa Flp-in cells treated with doxycycline to induce the equimolar expression of SSPB-GCN4-mVenus–ppKin14-VIb and iLID-mCherry-RAB11 (top; see also ) or of KIF1A(1–365)-VVDfast-mVenus-SSPB(micro) and iLID-mCherry-RAB11 (bottom; see also ) before or during illumination. Dots represent one cell and bars indicate mean and 95% confidence intervals. Dotted lines represent the mean and the mean ± two times the SD of control cells. Numbers show the percentage of responsive cells for each dataset. Data were from at least three experiments. Scale bars are 10 µm.

Journal: The Journal of Cell Biology

Article Title: An optimized toolbox for the optogenetic control of intracellular transport

doi: 10.1083/jcb.201907149

Figure Lengend Snippet: Application of the optimized optogenetic toolbox to populations of cells. (A) Constructs: use of a P2A self-cleaving peptide enables expression of iLID-mCherry-RAB11 with SSPB-Kin14-VIb or opto-kinesin-SSPB at equimolar levels from a single construct. (B) Immunoblot (IB) of whole-cell lysates from U2OS cells expressing the indicated proteins. Dotted lines indicate the excision of lanes. Exp. MW, expected molecular weight. (C–F) Fixed-cell imaging (C) and quantification (D–F) of iLID-mCherry-RAB11 and opto-kinesin KIF1A(1–365)-VVDfast-GFP-SSPB(micro) from a single plasmid in COS-7 cells in the dark or after illumination with blue light (100 µW cm −2 ). (D) Quantification showing the NPF of RAB11 intensity. (E) Graph showing normalized cellular expression levels of KIF1A(1–365)-VVDfast-GFP-SSPB(micro) and iLID-mCherry-RAB11 when expressed from a single plasmid (magenta) as in A or using two separate constructs for motor and cargo (gray). (F) Graph showing normalized cellular expression levels of KIF1A(1–365)-VVDfast-GFP-SSPB(micro) and the peripheral fraction of RAB11 per cell in the dark (gray) and after illumination (blue). (G and H) Fixed-cell imaging (G) and quantification (H) of iLID-mCherry-RAB11 and SSPB(micro) in COS-7 from a single plasmid in COS-7 cells in the dark or after illumination with blue light (100 µW cm −2 ). Graph in H shows the normalized perinuclear fraction of RAB11 intensity (mean RAB11 MTOC intensity/mean cellular RAB11 intensity). (I) Live-cell imaging of iLID-mCherry-RAB11 in HeLa Flp-in cells treated with doxycycline to induce the equimolar expression of SSPB-GCN4-mVenus–ppKin14-VIb and iLID-mCherry-RAB11 (top; see also ) or of KIF1A(1–365)-VVDfast-mVenus-SSPB(micro) and iLID-mCherry-RAB11 (bottom; see also ) before or during illumination. Dots represent one cell and bars indicate mean and 95% confidence intervals. Dotted lines represent the mean and the mean ± two times the SD of control cells. Numbers show the percentage of responsive cells for each dataset. Data were from at least three experiments. Scale bars are 10 µm.

Article Snippet: U2OS cells were transfected with iLID-mCherry-RAB11, KIF1A(1–365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11 or SSPB(micro)-mVenus-GCN4–ppKin14-VIb(861–1321)_P2A_iLID-mCherry-RAB11 using polyethylenimine (Polysciences).

Techniques: Construct, Expressing, Western Blot, Molecular Weight, Imaging, Plasmid Preparation, Live Cell Imaging

Application of the optimized optogenetic toolbox to endogenously tagged cargo. (A and B) Fixed-cell imaging (A) and quantification (B) of recycling endosomes, endogenously tagged with iLID-mCherry-RAB11 and transiently expressed SSPB(nano)-GFP-GCN4–ppKin14-VIb in HeLa cells, in the dark or after 30 min of blue light. Insets show 2.5-fold magnifications of the MTOC region. (B) Quantification shows the normalized enrichment of RAB11 at the MTOC relative to nonilluminated cells. Dots represent one cell and bars indicate mean and 95% confidence intervals. Data were from three independent experiments. Scale bars are 10 µm. Asterisks indicate significance (Student’s t test, unpaired). ∗∗∗∗, P < 0.0001.

Journal: The Journal of Cell Biology

Article Title: An optimized toolbox for the optogenetic control of intracellular transport

doi: 10.1083/jcb.201907149

Figure Lengend Snippet: Application of the optimized optogenetic toolbox to endogenously tagged cargo. (A and B) Fixed-cell imaging (A) and quantification (B) of recycling endosomes, endogenously tagged with iLID-mCherry-RAB11 and transiently expressed SSPB(nano)-GFP-GCN4–ppKin14-VIb in HeLa cells, in the dark or after 30 min of blue light. Insets show 2.5-fold magnifications of the MTOC region. (B) Quantification shows the normalized enrichment of RAB11 at the MTOC relative to nonilluminated cells. Dots represent one cell and bars indicate mean and 95% confidence intervals. Data were from three independent experiments. Scale bars are 10 µm. Asterisks indicate significance (Student’s t test, unpaired). ∗∗∗∗, P < 0.0001.

Article Snippet: U2OS cells were transfected with iLID-mCherry-RAB11, KIF1A(1–365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11 or SSPB(micro)-mVenus-GCN4–ppKin14-VIb(861–1321)_P2A_iLID-mCherry-RAB11 using polyethylenimine (Polysciences).

Techniques: Imaging

Spontaneous recovery from induced repositioning reveals transport dynamics of different organelles. (A) Assay. (B–J) Live-cell imaging (B, E, and H) and quantifications (C, D, F, G, I, and J) of LAMP1-mCherry-iLID (B–D), iLID-mCherry-RAB5 (E–G), or iLID-mCherry-RAB11 (H–J) in U2OS cells expressing opto-kinesin KIF1A(1–365)-VVDfast-GFP-SSPB(micro) or SSPB(micro)-GFP-GCN4-ppKin14-VIb before, during (middle), or 800 s after 200 s of illumination with blue light. Quantifications show change in peripheral organelle enrichment index (C, F, and I) or change in perinuclear enrichment index (D, G, and J). Graphs represent mean and SEM of at least three cells. Blue box indicates 470-nm illumination. Scale bars are 10 µm.

Journal: The Journal of Cell Biology

Article Title: An optimized toolbox for the optogenetic control of intracellular transport

doi: 10.1083/jcb.201907149

Figure Lengend Snippet: Spontaneous recovery from induced repositioning reveals transport dynamics of different organelles. (A) Assay. (B–J) Live-cell imaging (B, E, and H) and quantifications (C, D, F, G, I, and J) of LAMP1-mCherry-iLID (B–D), iLID-mCherry-RAB5 (E–G), or iLID-mCherry-RAB11 (H–J) in U2OS cells expressing opto-kinesin KIF1A(1–365)-VVDfast-GFP-SSPB(micro) or SSPB(micro)-GFP-GCN4-ppKin14-VIb before, during (middle), or 800 s after 200 s of illumination with blue light. Quantifications show change in peripheral organelle enrichment index (C, F, and I) or change in perinuclear enrichment index (D, G, and J). Graphs represent mean and SEM of at least three cells. Blue box indicates 470-nm illumination. Scale bars are 10 µm.

Article Snippet: U2OS cells were transfected with iLID-mCherry-RAB11, KIF1A(1–365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11 or SSPB(micro)-mVenus-GCN4–ppKin14-VIb(861–1321)_P2A_iLID-mCherry-RAB11 using polyethylenimine (Polysciences).

Techniques: Live Cell Imaging, Expressing